Aminoacyl-tRNA synthetases and aminoacylation of tRNA in the nucleus.
نویسنده
چکیده
This review is focused on findings concerning the presence of translation apparatus components (aminoacyl-tRNA synthetases, aminoacyl-tRNA, elongation factors) as well as translation itself in the nucleus. A nuclear role of these molecules is unknown. New findings suggest that well-accepted model of spatial segregation of transcription and translation in eukaryotic cell may be oversimplifcation. Nuclear coupling of both these processes show us how exciting and surprising may be the world of the living cell.
منابع مشابه
Active aminoacyl-tRNA synthetases are present in nuclei as a high molecular weight multienzyme complex.
Recent studies suggest that aminoacylation of tRNA may play an important role in the transport of these molecules from the nucleus to the cytoplasm. However, there is almost no information regarding the status of active aminoacyl-tRNA synthetases within the nuclei of eukaryotic cells. Here we show that at least 13 active aminoacyl-tRNA synthetases are present in purified nuclei of both Chinese ...
متن کاملRole of nuclear pools of aminoacyl-tRNA synthetases in tRNA nuclear export.
Reports of nuclear tRNA aminoacylation and its role in tRNA nuclear export (Lund and Dahlberg, 1998; Sarkar et al., 1999; Grosshans et al., 20001) have led to the prediction that there should be nuclear pools of aminoacyl-tRNA synthetases. We report that in budding yeast there are nuclear pools of tyrosyl-tRNA synthetase, Tys1p. By sequence alignments we predicted a Tys1p nuclear localization s...
متن کاملExpanding a tyrosyl-tRNA synthetase assay to other aminoacyl-tRNA synthetases
Aminoacyl-tRNA synthetases catalyze the attachment of amino acids to their cognate tRNAs. In general, aminoacyl-tRNA synthetase assays require stoichiometric amounts of tRNA, which limits their sensitivity while increasing their cost. This requirement for stoichiometric amounts of tRNA can be alleviated if the aminoacyl-tRNA product is cleaved following the tRNA aminoacylation reaction, regener...
متن کاملStudy of the role of the acceptor stem in the interactions between tRNAs and aminoacyl-tRNA synthetases.
Several studies have clearly demonstrated that the end of the acceptor stem was a very important area determining the aminoacylation properties of tRNAs. However the attempts to measure the contribution of this region to the binding of tRNAs to aminoacyl-tRNA synthetases have led to contradictory results. We report here the stepwise degradation of yeast tRNA-Phe and tRNA-Val from their 3' termi...
متن کاملFootprints of aminoacyl-tRNA synthetases are everywhere.
Aminoacyl-tRNA synthetases are at the center of the question of the origin of life 1,2. For that reason, proteins that resemble tRNA synthetases are of great interest. The large number of sequences of proteins emerging from the various genome projects has provided opportunity to search for new sequences that are 'synthetase-like'. Several have been identified and, although the functions of many...
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عنوان ژورنال:
- Acta biochimica Polonica
دوره 49 1 شماره
صفحات -
تاریخ انتشار 2002